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Assay Method Information

Assay Name:  DHODH Inhibition Assay
Description:  The assays were carried out by using a colorimetric DCIP method, which uses the colorimetric reagent 2, 6-dichlorophenolindophenol as the final electron acceptor. DCIP reduction is stoichiometrically equivalent to oxidation of dihydroorotate. Changes in absorbance were quantified in triplicate on a plate reader and data were analyzed to measure the reduction of DCIP as a decrease in absorbance at 600 nm. For the determination of the IC50 values (concentration of inhibitor required for 50% inhibition) eight different inhibitor concentrations were applied. Each data point was recorded in triplicates on a single measurement day. Data were analyzed with the program GraFit34 using a four-parameter fit.
Affinity data for this assay
 

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Last update November 1, 2007
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